Methods in Molecular Biology Quiz Solved. Recent BIO203 Quiz 1 Solution for Help in Studies or Exams and Improve Knowledge or Learning Skills. Also, Get PDF File Given Below.
BIO203 QUIZ 1 SOLVED
1. Allele-specific PCR uses primers whose ___ encompass the SNP
a) 3 ends
b) 5 ends
c) Promoter region
d) None
2. Inverse PCR is used for the identification and amplification of ___.
a) Long genes
b) Flanking sequence
c) Promoter region
d) Whole genome
3. Eco R1 is a ___ enzymes that can digest the genomic DNA
a) Hexa cutter
b) Tertra cutter
c) Penta cutter
d) Polycutter
4. In situ PCR is limited to the detection of ___ materials.
a) Genomic
b) Non-genomic
c) Both (a) and (b)
d) None of the these
5. ___ can also used for genotyping individuals for a large number of loci
a) RFLP
b) AFLP
c) Insitu PCR
d) All given
6. ___ PCR uses priers whose 3 ends encompass the SNP.
a) Reverse Transcriptase PCR
b) Assembly PCR
c) Ligation mediated PCR
d) Allele-Specific PCR
7. Treatment with sodium bisulfite converts unmethylated cytosine bases to ___.
a) Uracil
b) Thymine
c) Adenine
d) Guanine
8. Pfu polymerase enzyme is particularly used in ___ PCR.
a) Real-time PCR
b) Conventional PCR
c) RT-PCR
d) Long
9. The larger genes are cloned with the ___ PCR
a) Long
b) Conventional
c) RT-PCR
d) All
10. A fixed call or tissue can be amplified by ___ type of PCR
a) In situ
b) RT-PCT
c) Nested PCT
d) Colony PCR
11. Assembly PCR is used to assemble two or more pieces of DNA into ___.
a) One piece
b) Two piece
c) Three piece
d) None of these
12. ___ is used to assemble two or more pieces of DNA into one piece.
a) Colony PCR
b) Inverse PCR
c) Assembly PCR
d) All given
13. AFLP is a highly sensitive PCR-based method used for detecting polymorphisms in ___.
a) RNA
b) DNA
c) Both a and b
d) None of these
14. Suicide PCR is used in ___.
a) Cytogenetics
b) Human Genetics
c) Paleogenetics
d) None of these
15. Treatment with sodium bisulfite converts unmethylated cytosine bases to ___.
a) Uracil
b) Thymine
c) Adenine
d) Guanine
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